Čeština
EnglishToxic effect of nitroesters on plant tissue cultures
Podlipná R., Fialová Z., Vaněk T.
PLANT CELL, TISSUE AND ORGAN CULTURE 94 [3]: 305-311, 2008
Keywords: Nitroglycerine, Ethylene glycol dinitrate, Phytotoxicity, Phytoremediation
Abstract: Nitroglycerine (NG) is used in manufacture of dynamite, gunpowder, and rocket propellants, and as a therapeutic agent primarily to angina pectoris. Ethylene glycol dinitrate (EGDN) is an explosive ingredient (60–80%) in dynamite along with nitroglycerine (40–20%). Both compounds are pollutants, and due to recalcitrance to biodegradation they persist in the environment. In our study we concentrated on the phytotoxicity of these compounds, looking in particular at their effects on germination, growth and development. We used mustard (Sinapis alba) seedlings, in vitro cultivated regenerants of Juncus inflexus and Phragmites australis and suspension cultures of flax (Linum usitatissimum). The flax cells absorbed NG and transformed it to both dinitroglycerine isomers. By measuring their effect on the length of primary root of white mustard seedlings we demonstrated that the toxicity of these degradation products was lower than the original compound. The phytotoxic effect of EGDN on wetland plant species is described for the first time. High concentrations of EGDN (500 mg l−1 and higher) in the medium induced wilting and browning and subsequent death of reed and rush plants.
DOI:
Fulltext: contact IEB authors
IEB authors: Radka Podlipná, Tomáš Vaněk
PLANT CELL, TISSUE AND ORGAN CULTURE 94 [3]: 305-311, 2008
Keywords: Nitroglycerine, Ethylene glycol dinitrate, Phytotoxicity, Phytoremediation
Abstract: Nitroglycerine (NG) is used in manufacture of dynamite, gunpowder, and rocket propellants, and as a therapeutic agent primarily to angina pectoris. Ethylene glycol dinitrate (EGDN) is an explosive ingredient (60–80%) in dynamite along with nitroglycerine (40–20%). Both compounds are pollutants, and due to recalcitrance to biodegradation they persist in the environment. In our study we concentrated on the phytotoxicity of these compounds, looking in particular at their effects on germination, growth and development. We used mustard (Sinapis alba) seedlings, in vitro cultivated regenerants of Juncus inflexus and Phragmites australis and suspension cultures of flax (Linum usitatissimum). The flax cells absorbed NG and transformed it to both dinitroglycerine isomers. By measuring their effect on the length of primary root of white mustard seedlings we demonstrated that the toxicity of these degradation products was lower than the original compound. The phytotoxic effect of EGDN on wetland plant species is described for the first time. High concentrations of EGDN (500 mg l−1 and higher) in the medium induced wilting and browning and subsequent death of reed and rush plants.
DOI:
Fulltext: contact IEB authors
IEB authors: Radka Podlipná, Tomáš Vaněk