Čeština
EnglishRapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance
Kozak J, West CE, White C, da Costa-Nunes JA, Angelis KJ
DNA REPAIR 8: 413-9, 2009
Keywords: AtKU80, AtRAD21.1, AtLIG4-4, MIM, Bleomycin, Comet assay
Abstract: DNAdouble strand breaks (DSBs) are one of the most cytotoxic forms ofDNAdamage andmust be repaired by recombination, predominantly via non-homologous joining of DNA ends (NHEJ) in higher eukaryotes. However, analysis of DSB repair kinetics of plant NHEJ mutants atlig4-4 and atku80 with the neutral comet assay shows that alternative DSB repair pathways are active. Surprisingly, these kinetic measurements show that DSB repair was faster in the NHEJ mutant lines than in wild-type Arabidopsis. Herewe provide the first characterization of this KU-independent, rapid DSB repair pathway operating in Arabidopsis. The alternate pathway that rapidly removes the majority of DSBs present in nuclear DNA depends upon structural maintenance of chromosomes (SMC) complex proteins, namely MIM/AtRAD18 andAtRAD21.1.Anabsolute requirement forSMCproteins and kleisin for rapid repair of DSBs in Arabidopsis opens new insight into the mechanism of DSB removal in plants.
DOI:
IEB authors: Karel J. Angelis
DNA REPAIR 8: 413-9, 2009
Keywords: AtKU80, AtRAD21.1, AtLIG4-4, MIM, Bleomycin, Comet assay
Abstract: DNAdouble strand breaks (DSBs) are one of the most cytotoxic forms ofDNAdamage andmust be repaired by recombination, predominantly via non-homologous joining of DNA ends (NHEJ) in higher eukaryotes. However, analysis of DSB repair kinetics of plant NHEJ mutants atlig4-4 and atku80 with the neutral comet assay shows that alternative DSB repair pathways are active. Surprisingly, these kinetic measurements show that DSB repair was faster in the NHEJ mutant lines than in wild-type Arabidopsis. Herewe provide the first characterization of this KU-independent, rapid DSB repair pathway operating in Arabidopsis. The alternate pathway that rapidly removes the majority of DSBs present in nuclear DNA depends upon structural maintenance of chromosomes (SMC) complex proteins, namely MIM/AtRAD18 andAtRAD21.1.Anabsolute requirement forSMCproteins and kleisin for rapid repair of DSBs in Arabidopsis opens new insight into the mechanism of DSB removal in plants.
DOI:
Fulltext:
kozak_et_al_rapid_dsb_repai... (1.1 MB)