Čeština
EnglishGlutamate kinase as a potential biomarker of heavy metal stress in plants
Pavlíková D., Pavlík M., Staszková L., Motyka V., Száková J., Tlustoš P., Balík J.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 70: 223-230, 2008
Keywords: Cadmium; Chronic stress; Glutamate kinase [E.C.2.7.2.11]; Proline; Spinacia oleracea L.; Spinach; Zinc
Abstract: Changes of glutamate kinase activity (GKA) in plants under cadmium and zinc chronic stress reported here reveal a regulatory role of this enzyme in plant heavy metal stress adaptation and indicate its potential use as a stress biomarker. Results of the first experimental series confirmed the toxic effects of cadmium and zinc at tested levels (30, 60, 90 mgCd kg-1 and 250, 500, 750mg Zn kg-1 soil) for spinach. A significant decrease of GKA in plants grown on contaminated treatments was found. Changes of GKA of plants grown on the highest contaminated treatments in the second series of experiments showed a similar course as a curve of plant stress response indicating the process of plant adaptation to chronic stresses—the decline of GKA in period of damage of cell activities, increase of its activity in period of maximum resistance and its following decrease in period of the plant metabolism depletion.
DOI:
Fulltext: contact IEB authors
IEB authors: Václav Motyka, Milan Pavlík
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 70: 223-230, 2008
Keywords: Cadmium; Chronic stress; Glutamate kinase [E.C.2.7.2.11]; Proline; Spinacia oleracea L.; Spinach; Zinc
Abstract: Changes of glutamate kinase activity (GKA) in plants under cadmium and zinc chronic stress reported here reveal a regulatory role of this enzyme in plant heavy metal stress adaptation and indicate its potential use as a stress biomarker. Results of the first experimental series confirmed the toxic effects of cadmium and zinc at tested levels (30, 60, 90 mgCd kg-1 and 250, 500, 750mg Zn kg-1 soil) for spinach. A significant decrease of GKA in plants grown on contaminated treatments was found. Changes of GKA of plants grown on the highest contaminated treatments in the second series of experiments showed a similar course as a curve of plant stress response indicating the process of plant adaptation to chronic stresses—the decline of GKA in period of damage of cell activities, increase of its activity in period of maximum resistance and its following decrease in period of the plant metabolism depletion.
DOI:
Fulltext: contact IEB authors
IEB authors: Václav Motyka, Milan Pavlík