Čeština
EnglishARP2 and ARP3 are localized to sites of actin filament nucleation in tobacco BY-2 cells
Fišerová, J.; Schwarzerová, K.; Petrášek, Jan; Opatrný, Z.
PROTOPLASMA 227 [2-4]: 119-128, 2006
Keywords: Nicotiana tabacum; BY-2 cells; actin filament
Abstract: Complete depolymerization of actin filaments (AFs) at low temperature (0 C) is followed by the formation of transient actin structures at 25 C in tobacco BY-2 cells (Nicotiana tabacum L.). Using antibodies against fission yeast actin-related proteins (ARP2 and ARP3). we show here that transient actin structures (dots. dotted filaments. rods) colocalize with epitopes stained by these antibodies and thus are likely to represent sites of actin filament nucleation (SANs). In contrast to the cold-induced disassembly of AFs, no transient actin structures were detectable during recovery of AFs from latrunculin B-induced depolymerization. However, the staining pattern obtained with ARP antibodies in latrunculin B-treated cells was similar to that in controls and cold-treated cells. This suggests that, in addition to the complete depolymerization of AFs. disruption of other cellular structures is needed for the formation of transient actin structures during the early phase of recovery from cold treatment.
DOI:
IEB authors: Jan Petrášek
PROTOPLASMA 227 [2-4]: 119-128, 2006
Keywords: Nicotiana tabacum; BY-2 cells; actin filament
Abstract: Complete depolymerization of actin filaments (AFs) at low temperature (0 C) is followed by the formation of transient actin structures at 25 C in tobacco BY-2 cells (Nicotiana tabacum L.). Using antibodies against fission yeast actin-related proteins (ARP2 and ARP3). we show here that transient actin structures (dots. dotted filaments. rods) colocalize with epitopes stained by these antibodies and thus are likely to represent sites of actin filament nucleation (SANs). In contrast to the cold-induced disassembly of AFs, no transient actin structures were detectable during recovery of AFs from latrunculin B-induced depolymerization. However, the staining pattern obtained with ARP antibodies in latrunculin B-treated cells was similar to that in controls and cold-treated cells. This suggests that, in addition to the complete depolymerization of AFs. disruption of other cellular structures is needed for the formation of transient actin structures during the early phase of recovery from cold treatment.
DOI: