Dobesova M., Kolackova M., Pencik O., Capal P., Chaloupsky P., Svec P., Ridoskova A., Motola M., Cicmancova V., Sopha H., Macak J.M., Richtera L., Adam V., Huska D.
AQUATIC TOXICOLOGY 256: 106419, 2023

Klíčová slova:
Abstrakt: Recently, more accessible transcriptomic approaches have provided a new and deeper understanding of environmental toxicity. The present study focuses on the transcriptomic profiles of green microalgae Chlamydomonas reinhardtii exposed to new industrially promising material, TiO2 nanotubes (NTs), as an example of a widely used one-dimensional nanomaterial. The first algal in vitro assay included 2.5 and 7.5 mg/L TiO2 NTs, resulting in a dose-dependent negative effect on biological endpoints. At a working concentration of 7.5 mg/L, RNAsequencing showed a mainly negative effect on the cells. In summary, the results indicated metabolic disruption, such as ATP loss, damage to mitochondria and chloroplasts, loss of solutes due to permeated membranes, and cell wall damage. Moreover, apoptosis-induced transcripts were detected. Interestingly, reactivation of transposons was observed. In signalling and transcription pathways, including chromatin remodelling and locking, the annotated genes were downregulated.
DOI: 10.1016/j.aquatox.2023.106419
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