Denysiuk K.V., Satarova T.M., Semenova V.V., Cherchel V.Y., Soudek P., Dziubets'kiy B.
AGRICULTURAL SCIENCE AND PRACTICE 11(1): 77-85, 2024

Klíčová slova: Zea mays L., beta-carotene hydroxylase 1, single nucleotide polymorphism, molecular markers, carotenoids
Abstrakt: SNP analysis and estimation of genetic relations in maize inbreds with the alternative state of beta-carotene hydroxylase 1 gene (crtRB1) by the molecular marker crtRB1-3'TE. Methods. SNP analysis, statistical methods. BDIIII panel with 384 SNP markers was used for comparative research of 35 maize inbreds developed and adopted in the northern Steppe of Ukraine. Results. SNP analysis of investigated inbred set showed that the part of dimorphic markers was 98.2 %, the average minor allele frequency (MAF) was 0.3040, the average polymorphism information content (PIC) was 0.3064, and the average shift of genetic diversity of markers was 0.3898. There was no significant difference between genetic SNP distances within both groups of inbreds with favourable/unfavourable alleles and between these groups by crtRB1-3'TE. The SNP distances were used to build a dendrogram of genetic relations between maize inbreds with an alternative state of the crtRB1-3'TE marker. Conclusions. The relationship between the allelic state of the beta-carotene hydroxylase 1 gene and single nucleotide polymorphism markers for maize inbreds is presented. The alleles of SNP markers BDI-III-130A, BDI-III-15A, BDI-III-60C, BDI-III-61C, BDI-III-116G, BDIIII-128A, and BDI-III-129. were found to be most frequent in the inbreds, which are carriers of the favourable allele of 543 bp of crtRB1 gene by the crtRB1-3'TE marker. The random distribution of alleles of this gene among maize inbreds developed in the northern Steppe of Ukraine was demonstrated.
DOI: 10.15407/agrisp11.01.077 Autoři z ÚEB: Tetiana Satarova, Petr Soudek