Čeština
EnglishExpression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production
Plchova H., Moravec T., Dedic P., Cerovska N.
JOURNAL OF PHYTOPATHOLOGY 159: 130-132, 2011
Klíčová slova: Potato leafroll virus, recombinant viral antigen, antibody production
Abstrakt: Vector pMPM-A4omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)–fragments RdRp43-616 and RdRp304-537 were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Noemi Čeřovská, Tomáš Moravec, Helena Plchová
JOURNAL OF PHYTOPATHOLOGY 159: 130-132, 2011
Klíčová slova: Potato leafroll virus, recombinant viral antigen, antibody production
Abstrakt: Vector pMPM-A4omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)–fragments RdRp43-616 and RdRp304-537 were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Noemi Čeřovská, Tomáš Moravec, Helena Plchová