Čeština
English24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA
Franěk, František; Eckschlager, T.; Kohout, Ladislav
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 68 [11]: 2190-2200, 2003
Klíčová slova: Steroids; Brassinosteroids; 24-Epibrassinolides
Abstrakt: A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to significant changes in cell culture parameters.
DOI:
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COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS 68 [11]: 2190-2200, 2003
Klíčová slova: Steroids; Brassinosteroids; 24-Epibrassinolides
Abstrakt: A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to significant changes in cell culture parameters.
DOI: