Centre of Plant Structural and Functional Genomics

Contacts
Group head: 
Jan Bartoš
Centre: 
Olomouc
Address: 
Šlechtitelů 31, 783 71, Olomouc - Holice, Česká republika
Email: 
bartosatueb [dot] cas [dot] cz
Phone: 
+420 585 238 711
www: 
Centre of Plant Structural and Functional Genomics

Research Topics

The Centre of Plant Structural and Functional Genomics has been established to study the structure and function of nuclear genome in higher plants. The main focus is on economically important crops like cereals, legumes, bananas and plantains.

Methodological approaches involve classical and molecular cytogenetics, flow cytometry and sorting, recombinant DNA techniques and construction of chromosome and chromosome-arm specific BAC libraries. Because of the complexity of research projects, the Laboratory collaborates with many research groups throughout the world. The laboratory staff is also involved in teaching and technology transfer, including consultancy services in the above mentioned areas.

Flow cytogenetics

Flow cytogenetics involve chromosome analysis and sorting using flow cytometry applications of sorted chromosomes include physical mapping using PCR, targeted isolation of molecular markers, isolation of low-copy (genic) sequences from specific genome regions, high-throughput physical mapping of DNA sequences by hybridization to DNA arrays, as well as HAPPY and optical mapping.

Chromosome-based BAC libraries recources

The most attractive use of sorted chromosomes is the production of chromosome- and chromosome arm-specific DNA libraries. Such libraries provide a permanent and easily accessible molecular resource specific for a particular part of the genome, is expected to have a major impact on wheat genomics.

Flow cytometry

Flow cytometry is powerful technique with many application. We used it for genome size estimation, ploidy measurement and flow karyotype analysis of higher plants.

Banana studies

We interested in molecular evolution and organization of Musa genome and serve as the Musa Genome Resources Centre (MGRC). The primary aim of the Centre is to support research activities of the Musa Genomics Consortium by distributing Musa genome resources to Consortium Members and by developing new resources.

Study on Festuca-Lolium complex

We are using molecular cytogenetic methods (FISH and GISH) to study genomic constitution of Festuca × Lolium hybrids, chromosome pairing during meiosis, and to map BAC clones on chromosomes of Festuca pratensis. In collaboration with other labs, we also developed set of introgression lines of all F. pratensis chromosomes in tetraploid L. multiflorum and DArT chip for five species within Festuca-Lolium complex.

Workers: 

head of the laboratory
senior scientist

deputy head of the laboratory
senior scientist

project manager

senior scientist

scientist

scientific assistant

graduated technical assistant

THS employee

pracovník THS

postdoc

postgraduate student

assistant

technician

maintenance

gatekeeper

Research projects: 
Publications: 
Number of publications: 563

2018

  • Rht18 semidwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content
    Ford, B.A., Foo, E., Sharwood, R., Karafiátová, M., Vrána, J., MacMillan, C., Nichols, D.S., Steuernagel, B., Uauy, C., Doležel, J., Chandler, P.M., Spielmeyer, W.
    PLANT PHYSIOLOGY 177 168-180 2018 Fulltext: PDF link on intranet only
  • Sequence divergence between spelt and common wheat
    Liu, M., Zhao, Q., Qi, F., Stiller, J., Tang, S., Miao, J., Vrána, J., Holušová, K., Liu, D., Doležel, J., Manners, J.M., Han, B., Liu, C.
    THEORETICAL AND APPLIED GENETICS 131 1125-1132 2018 Fulltext: PDF link on intranet only
  • Pm21 from Haynaldia villosa encodes a CC-NBS-LRR protein conferring powdery mildew resistance in wheat
    Xing, L., Hu, P., Liu, J., Witek, K., Zhou, S., Xu, J., Zhou, W., Gao, L., Huang, Z., Zhang, R., Wang, X., Chen, P., Wang, H., Jones, J.D.G., Karafiátová, M., Vrána, J., Bartoš, J., Doležel, J., Tian, Y., Wu, Y., Cao, A.
    MOLECULAR PLANT 11 874-878 2018 Fulltext: PDF link on intranet only
  • Integration of genetic and cytogenetic maps and identification of sex chromosome in garden asparagus (Asparagus officinalis L.)
    Moreno, R., Castro, P., Vrána, J., Kubaláková, M., Cápal, P., García, V., Gil, J., Millán, T., Doležel, J.
    FRONTIERS IN PLANT SCIENCE 9 1068 2018 Fulltext: PDF link on intranet only
  • Large-scale structural variation detection in subterranean clover subtypes using optical mapping
    Yuan, Y., Milec, Z., Bayer, P.E., Vrána, J., Doležel, J., Edwards, D., Erskine, W., Kaur, P.
    FRONTIERS IN PLANT SCIENCE 9 971 2018 Fulltext: PDF link on intranet only
  • Shifting the limits in wheat research and breeding using a fully annotated reference genome
    International Wheat Genome Sequencing Consortium (IWGSC); IWGSC RefSeq principal investigators: Appels, R., Eversole, K., Feuillet, C., Keller, B., Rogers, J., Stein, N.; IWGSC whole-genome assembly principal investigators: Pozniak, C.J., Stein, N., Choulet, F., Distelfeld, A., Eversole, K., Poland, J., Rogers, J., Ronen, G., Sharpe, A.G.; Whole-genome sequencing and assembly: Pozniak, C., Ronen, G., Stein, N., Barad, O., Baruch, K., Choulet, F., Keeble-Gagnère, G., Mascher, M., Sharpe, A.G., Ben-Zvi, G., Josselin, A.A.; Hi-C data-based scaffolding: Stein, N., Mascher, M., Himmelbach, A.; Whole-genome assembly quality control and analyses: Choulet, F., Keeble-Gagnère, G., Mascher, M., Rogers, J., Balfourier, F., Gutierrez-Gonzalez, J., Hayden, M., Josselin, A.A., Koh, C., Muehlbauer, G., Pasam, R.K., Paux, E., Pozniak, C.J., Rigault, P., Sharpe, A.G., Tibbits, J., Tiwari, V.; Pseudomolecule assembly: Choulet, F., Keeble-Gagnère, G., Mascher, M., Josselin, A.A., Rogers, J.; RefSeq genome structure and gene analyses: Spannagl, M., Choulet, F., Lang, D., Gundlach, H., Haberer, G., Keeble-Gagnère, G., Mayer, K.F.X., Ormanbekova, D., Paux, E., Prade, V., Šimková, H., Wicker, T.; Automated annotation: Choulet, F., Spannagl, M., Swarbreck, D., Rimbert, H., Felder, M., Guilhot, N., Gundlach, H., Haberer, G., Kaithakottil, G., Keilwagen, J., Lang, D., Leroy, P., Lux, T., Mayer, K.F.X., Twardziok, S., Venturini, L.; Manual gene curation: Appels, R., Rimbert, H., Choulet, F., Juhász, A., Keeble-Gagnère, G.; Subgenome comparative analyses: Choulet, F., Spannagl, M., Lang, D., Abrouk, M., Haberer, G., Keeble-Gagnère, G., Mayer, K.F.X., Wicker, T.; Transposable elements: Choulet, F., Wicker, T., Gundlach, H., Lang, D., Spannagl, M.; Phylogenomic analyses: Lang, D., Spannagl, M., Appels, R., Fischer, I.; Transcriptome analyses and RNA-seq data: Uauy, C., Borrill, P., Ramirez-Gonzalez, R.H., Appels, R., Arnaud, D., Chalabi, S., Chalhoub, B., Choulet, F., Cory, A., Datla, R., Davey, M.W., Hayden, M., Jacobs, J., Lang, D., Robinson, S.J., Spannagl, M., Steuernagel, B., Tibbits, J., Tiwari, V., van Ex, F., Wulff, B.B.H.; Whole-genome methylome: Pozniak, C.J., Robinson, S.J., Sharpe, A.G., Cory, A.; Histone mark analyses: Benhamed, M., Paux, E., Bendahmane, A., Concia, L., Latrasse, D.; BAC chromosome MTP IWGSC–Bayer Whole-Genome Profiling (WGP) tags: Rogers, J., Jacobs, J., Alaux, M., Appels, R., Bartoš, J., Bellec, A., Berges, H., Doležel, J., Feuillet, C., Frenkel, Z., Gill, B., Korol, A., Letellier, T., Olsen, O.A., Šimková, H., Singh, K., Valárik, M., van der Vossen, E., Vautrin, S., Weining, S.; Chromosome LTC mapping and physical mapping quality control: Korol, A., Frenkel, Z., Fahima, T., Glikson, V., Raats, D., Rogers, J.; RH mapping: Tiwari, V., Gill, B., Paux, E., Poland, J.; Optical mapping: Doležel, J., Číhalíková, J., Šimková, H., Toegelová, H., Vrána, J.; Recombination analyses: Sourdille, P., Darrier, B.; Gene family analyses: Appels, R., Spannagl, M., Lang, D., Fischer, I., Ormanbekova, D., Prade, V.; CBF gene family: Barabaschi, D., Cattivelli, L.; Dehydrin gene family: Hernandez, P., Galvez, S., Budak, H.; NLR gene family: Steuernagel, B., Jones, J.D.G., Witek, K., Wulff, B.B.H., Yu, G.; PPR gene family: Small, I., Melonek, J., Zhou, R.; Prolamin gene family: Juhász, A., Belova, T., Appels, R., Olsen, O.A.; WAK gene family: Kanyuka, K., King, R.; Stem solidness (SSt1) QTL team: Nilsen, K., Walkowiak, S., Pozniak, C.J., Cuthbert, R., Datla, R., Knox, R., Wiebe, K., Xiang, D.; Flowering locus C (FLC) gene team: Rohde, A., Golds, T.; Genome size analysis: Doležel, J., Čížková, J., Tibbits, J.; MicroRNA and tRNA annotation: Budak, H., Akpinar, B.A., Biyiklioglu, S.; Genetic maps and mapping: Muehlbauer, G., Poland, J., Gao, L., Gutierrez-Gonzalez, J., N'Daiye, A.; BAC libraries and chromosome sorting: Doležel, J., Šimková, H., Číhalíková, J., Kubaláková, M., Šafář, J., Vrána, J.; BAC pooling, BAC library repository, and access: Berges, H., Bellec, A., Vautrin, S.; IWGSC sequence and data repository and access: Alaux, M., Alfama, F., Adam-Blondon, A.F., Flores, R., Guerche, C., Letellier, T., Loaec, M., Quesneville, H.; Physical maps and BAC-based sequences: 1A BAC sequencing and assembly: Pozniak, C.J., Sharpe, A.G., Walkowiak, S., Budak, H., Condie, J., Ens, J., Koh, C., Maclachlan, R., Tan, Y., Wicker, T.; 1B BAC sequencing and assembly: Choulet, F., Paux, E., Alberti, A., Aury, J.M., Balfourier, F., Barbe, V., Couloux, A., Cruaud, C., Labadie, K., Mangenot, S., Wincker, P.; 1D, 4D, and 6D physical mapping: Gill, B., Kaur, G., Luo, M., Sehgal, S.; 2AL physical mapping: Singh, K., Chhuneja, P., Gupta, O.P., Jindal, S., Kaur, P., Malik, P., Sharma, P., Yadav, B.; 2AS physical mapping: Singh, N.K., Khurana, J., Chaudhary, C., Khurana, P., Kumar, V., Mahato, A., Mathur, S., Sevanthi, A., Sharma, N., Tomar, R.S.; 2B, 2D, 4B, 5BL, and 5DL IWGSC–Bayer Whole-Genome Profiling (WGP) physical maps: Rogers, J., Jacobs, J., Alaux, M., Bellec, A., Berges, H., Doležel, J., Feuillet, C., Frenkel, Z., Gill, B., Korol, A., van der Vossen, E., Vautrin, S.; 3AL physical mapping: Gill, B., Kaur, G., Luo, M., Sehgal, S.; 3DS physical mapping and BAC sequencing and assembly: Bartoš, J., Holušová, K., Plíhal, O.; 3DL BAC sequencing and assembly: Clark, M.D., Heavens, D., Kettleborough, G., Wright, J.; 4A physical mapping, BAC sequencing, assembly, and annotation: Valárik, M., Abrouk, M., Balcárková, B., Holušová, K., Hu, Y., Luo, M.; 5BS BAC sequencing and assembly: Salina, E., Ravin, N., Skryabin, K., Beletsky, A., Kadnikov, V., Mardanov, A., Nesterov, M., Rakitin, A., Sergeeva, E.; 6B BAC sequencing and assembly: Handa, H., Kanamori, H., Katagiri, S., Kobayashi, F., Nasuda, S., Tanaka, T., Wu, J.; 7A physical mapping and BAC sequencing: Appels, R., Hayden, M., Keeble-Gagnère, G., Rigault, P., Tibbits, J.; 7B physical mapping, BAC sequencing, and assembly: Olsen, O.A., Belova, T., Cattonaro, F., Jiumeng, M., Kugler, K., Mayer, K.F.X., Pfeifer, M., Sandve, S., Xun, X., Zhan, B.; 7DS BAC sequencing and assembly: Šimková, H., Abrouk, M., Batley, J., Bayer, P.E., Edwards, D., Hayashi, S., Toegelová, H., Tulpová, Z., Visendi, P.; 7DL physical mapping and BAC sequencing: Weining, S., Cui, L., Du, X., Feng, K., Nie, X., Tong, W., Wang, L.; Figures: Borrill, P., Gundlach, H., Galvez, S., Kaithakottil, G., Lang, D., Lux, T., Mascher, M., Ormanbekova, D., Prade, V., Ramirez-Gonzalez, R.H., Spannagl, M., Stein, N., Uauy, C., Venturini, L.; Manuscript writing team: Stein, N., Appels, R., Eversole, K., Rogers, J., Borrill, P., Cattivelli, L., Choulet, F., Hernandez, P., Kanyuka, K., Lang, D., Mascher, M., Nilsen, K., Paux, E., Pozniak, C.J., Ramirez-Gonzalez, R.H., Šimková, H., Small, I., Spannagl, M., Swarbreck, D., Uauy, C.
    SCIENCE 361 7191 2018 Fulltext: PDF link on intranet only
  • Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome
    Keeble-Gagnère, G., Rigault, P., Tibbits, J., Pasam, R., Hayden, M., Forrest, K., Frenkel, Z., Korol, A., Huang, B.E., Cavanagh, C., Taylor, J., Abrouk, M., Sharpe, A., Konkin, D., Sourdille, P., Darrier, B., Choulet, F., Bernard, A., Rochfort, S., Dimech, A., Watson-Haigh, N., Baumann, U., Eckermann, P., Fleury, D., Juhasz, A., Boisvert, S., Nolin, M.A., Doležel, J., Šimková, H., Toegelová, H., Šafář, J., Luo, M.C., Câmara, F., Pfeifer, M., Isdale, D., Nyström-Persson, J., IWGSC, Koo, D.H., Tinning, M., Cui, D., Ru, Z., Appels, R.
    GENOME BIOLOGY 19 112 2018 Fulltext: PDF link on intranet only
  • Genotyping of Festulolium cultivars involved in EUCARPIA multi-site trial using DArT markers and GISH
    Kopecký, D., Baert, J., Barth, S., Bartoš, J., Černoch, V., Doležel, J., Grogan, D., Harper, J., Humphreys, M., Książczyk, T., Østrem, L., Paszkowski, E., Sokolovič, D., Zwierzykowski, Z., Ghesquière, M.
    Breeding grasses and protein crops in the era of genomics 155-159 2018 Fulltext: PDF link on intranet only
  • Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat
    Rey, E., Abrouk, M., Keeble-Gagnère, G., Karafiátová, M., Vrána, J., Balzergue, S., Soubigou-Taconnat, L., Brunaud, V., Martin-Magniette, M.L., Endo, T.R., Bartoš, J.; International Wheat Genome Sequencing Consortium (IWGSC), Appels, R., Doležel, J.
    PLANT BIOTECHNOLOGY JOURNAL 16 1767-1777 2018 Fulltext: PDF link on intranet only
  • Genome size in some taxa of Crepis L. (Asteraceae) from Turkey
    Inceer, H., Kalmuk, N.A., Čížková, J., Doležel, J.
    CARYOLOGIA 71 217-223 2018 Fulltext: PDF link on intranet only
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